بررسی اثر حذف برگشت پذیر هسته‌ قاعده‌ای‌ جانبی‌ آمیگدال‌ در فرآیند حافظه‌ فضایی‌ وابسته به هیپوکمپ در موش‌ آزمایشگاهی ‌

Introduction: Many evidences have suggested that the Basolateral Amygdala (BLA) are probably involved in emotional learning and modulation of spatial memory processes. The aim of this present study was assessment of the effect of reversible abolition of BLA on spatial memory processes in a place avoidance learning model in a stable environment. Methods and Materials: Long-Evans strain rats (280-320 gr.) were selected and cannulae aimed at the BLA were surgically implanted bilaterally. The mice were trained to avoid a 60° segment of the arena by punishing with a mild foot shock upon entering the area. The punished sector was defined by room cues during the place avoidance training, which occurred in a single 30-min session and the avoidance memory was assessed during a 30-min extinction trial after 24 hours. The time of the first entry and the number of entrances into the punished sector during extinction were used to measure the place avoidance memory. Bilateral injections of Tetrodotoxin (5ng/0.6ml per side) were used to inactivate the BLA 60 min before acquisition, immediately, 60 and 120 min after training, or 60 min before the retrieval test. Control mice were injected saline at the same time. Results : The results indicated that acquisition, consolidation (immediately, 60 min after training) and retrieval of spatial memory in stable arena were impaired (p0.05). Conclusion: We conclude that the Basolateral Amygdala (BLA) modulate spatial memory processes in place avoidance learning model in stable arena and this effect in regard to consolidation is time dependent.